Inhibitory effects of Coptis chinensis extract on the growth and biofilm formation of Streptococcus mutans and Streptococcus sobrinus
김시영, 송유리, 이현아, 나희삼, 정철종, Bek Gyung-Yun, 정진,
소속 상세정보
김시영 ( Kim Si-Yeong ) - Pusan National University School of Dentistry Department of Oral Microbiology
송유리 ( Song Yu-Ri ) - Pusan National University School of Dentistry Department of Oral Microbiology
이현아 ( Lee Hyun-Ah ) - Pusan National University School of Dentistry Department of Oral Microbiology
나희삼 ( Na Hee-Sam ) - Pusan National University School of Dentistry Department of Oral Microbiology
정철종 ( Jung Chul-Jong ) - Okchundang Research Institute
( Bek Gyung-Yun ) - Okchundang Research Institute
정진 ( Chung Jin ) - Pusan National University School of Dentistry Department of Oral Microbiology
Abstract
Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1?2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract’s inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus . Therefore, C. chinensis might be a potential candidate for controlling dental caries.
키워드
Coptis; Growth; Biofilm; Streptococcus mutans; Streptococcus sobrinus
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